西安荷斯坦奶牛群5个基因座位遗传多态性的PCR-RFLP分析

Analysis of Genetic Polymorphisms at Five Loci in Xi’an Population of Chinese Holstein Cattle by PCR-RFLP

应用PCR RFLP方法对西安荷斯坦牛的κcn、βlg、βlg 5′侧翼区、CSN1S2、IGFBP 3 共5 个基因座位进行了多态性分析。结果表明,在西安荷斯坦牛群中,没有发现携带CSN1S2F 等位基因的个体,其多态信息含量为0。κcn基因座位呈现低度多态(PIC=0 236 6),βlg、βlg 5′侧翼区、IGFBP 3 基因座位的多态信息含量分别为0 316 8、0 368 9、0 443 9,均呈现中度多态。κcn、βlg、βlg 5′侧翼区、IGFBP 3、CSN1S2 基因座位的杂合度和DNA多态度分别为0 274 2、0 394 7、0 487 9、0 489 1、0和0 025 5、0 011 6、0 033 3、0 011 2、0。而且,在西安荷斯坦牛群中,κcn、βlg、βlg 5′侧翼区、IGFBP 3 共4 个基因座位均处于Hardy Weinberg平衡状态,CSN1S2 基因座位处于纯合状态。

The genetic polymorphisms of Xi’an population of Chinese Holstein cattle were detected for κ-cn, β-lg, β-lg 5′flanking region, CSN1S2 F and IGFBP-3 genes by PCR-RFLP. No carriers of the allele of CSN1S2 F were found and its polymorphism information content was 0. κ-cn locus showed low polymorphism (PIC=0.236 6), the three loci of β-lg, β-lg 5′flanking region and IGFBP-3 genes moderate polymorphisms (0.316 8, 0.368 9 and 0.443 9 respectively). The heterozygosity (h) and DNA polymorphisms of the five loci (κ-cn,β-lg,β-lg 5′flanking region, IGFBP-3 and CSN1S2 F genes ) were 0.274 2, 0.394 7, 0.487 9, 0.489 1, 0.000 0 and 0.025 5, 0.011 6, 0.033 3, 0.011 2, 0.000 0 respectively. And κ-cn, β-lg, β-lg 5′flanking region and IGFBP-3 loci in the population were at Hardy-Weinberg equilibrium. Only BB genotype was found at CSN1S2 F loci.