体外诱导的成骨细胞与金属材料界面的初步研究

Inducement and Proliferation of MSCs in vitro and Preliminary Study on Interface between MSCs and Metal Materials

目的研究体外诱导成骨细胞的特性及其与金属材料贴附形成的界面。方法采用成年犬骨髓基质干细胞进行体外成骨诱导培养并置入金属材料,借助相差显微镜、生化检测、透射电镜、扫描电镜等手段进行分析。结果体外成骨诱导的骨髓基质干细胞显示出强大的成骨活性,并无论在光滑表面还是粗糙表面都能与材料贴附形成较好界面,且在应变量最大的张应力区分布较紧密。结论经诱导的骨髓基质干细胞可作为成骨细胞的来源,并能在金属材料表面贴附形成界面,且实验所采用的喷砂粗化处理对其影响并不显著,但材料的应变程度及应力性质可能与之有一定的关系。

Objective The aims of the present study were to assess the characteristics of MSCs which were induced and proliferated in vitro, then to learn the interfaces between them and metal materials.Methods Adult dogs were selected as experimental animals, the MSCs (marrow stem cells) were aspirated from the femur, which were induced to the phenotype of OB (osteoblast), then to proliferated in vitro with metal materials. Using biochemical tests, phase contrast microscopy, transmit electromicroscope, scanning electromicroscope to detect MSCs and the image of interface.Results MSCs had powerful osteogenic phenotype when they were induced in vitro, and they also attached to the metal materials. And there were more cells which attached on tensile stress area than other areas.Conclusion The MSCs induced in vitro could be used as source of osteoblast. The affects of air-abrasion with alumina are not significant, but they have relation to the extent of strain and property of stress.