性传播感染人群和普通人群几种少见支原体的检测

Detection of several rare kinds of Mycoplasmas in patients with sexually transmitted infections and in general population

目的了解性传播感染(STI)人群和普通人群中几种少见支原体感染状况。方法用套式PCR(NestedPCR)方法对STI人群和普通人群的尿道(宫颈)分泌物进行生殖支原体(Mg)、穿通支原体(Mpe)、梨支原体(Mpi)和发酵支原体(Mf)的检测。结果STI人群的阳性率分别为Mg19.32%(1788)、Mpe9.09%(888)、Mpi2.27%(288)和Mf0(088)。普通人群的阳性率分别为Mg0.58%(1171)、Mpe0(0171)、Mpi1.17%(2171)和Mf1.75%(3171),统计学分析表明,两组中Mg(χ2=36.14,P<0.0001)、Mpe(P<0.0001)阳性率有显著差异,提示在STI人群中Mg和Mpe的检出率显著高于普通人群,而Mpi和Mf无显著性差异(P>0.05)。男性STI人群中Mg、Mpe检出率显著高于普通人群(P<0.001),Mpi无显著性差异(P>0.05)。在女性STI人群中,Mg检出率也非常显著高于普通人群(P<0.001),但Mpe、Mpi、Mf在两个人群中均无显著性差异(P>0.05)。普通人群中男性均未检出Mg、Mpe、Mpi和Mf。结论Mg、Mpe可能通过性接触传播,与尿道炎和宫颈炎有一定相关性,对STI人群中的Mg、Mpe感染问题应作进一步的研究。

Objective: To determine the prevalence of Mycoplasmas in patients with sexually transmitted infections and in general population. Methods: Nested PCR was used to detect M. genitaliun (Mg), M. penetrate (Mpe), M. fermentants (Mf) and M. pirum (Mpi) in urethral or cervical specimens collected from 88 (male 53, female 35) patients with sexually transmitted infections (STI) and 171 (male 99, female 72) individuals from general population. Results: In STI patients, the detection rates of Mg, Mpe, Mf and Mpi were 19.32% (17/88), ~9.09% (8/88), 2.27 (2/88) and 0(0/88) respectively. In general group, the prevalence of these four mycoplasmas was Mg 0.58% (1/171), Mpe 0(0/171), Mpi 1.17% (2/171), and Mf 1.75% (3/171). The infection of Mg and Mpe in STI patients was much higher than that in general population (P<0.001). However, there was no difference in Mpi and Mf infection between STI group and general population (P>0.05). In males, the detection rates of Mpe and Mg were higher in STI patients than those in general population (P<0.001), but there was no difference in Mpi detection rate between the two groups (P>0.05). In females, the detection rate of Mg was significantly higher in STI than that in general population (P<0.01), yet the prevalence of Mpe, Mpi and Mf did not significantly differed between these two groups. The detection of Mg, Mpe, Mpi and Mf in general male individuals was negative. ~Conclusion : Mg and Mpe can be transmitted by sexual activities and there is an association of Mg and Mpe with NGU and cervicitis.