摘要
以体外转录合成、同位素35S标记的肠道病毒特异负股RNA为探针,对急性柯萨奇B3病毒(CVB3)心肌炎小鼠心肌中的CVB3-RNA进行RNA-RNA原位杂交检测,利用图像分析仪对阳性杂交信号进行图像处理与定量分析;观察黄芪对CVB3-RNA含量的影响;同时对黄芪的抗病毒机理及其与β-干扰素的关系进行了初步探讨。结果发现在病毒感染的心肌组织中,黄芪组心肌坏死面积明显小于生理盐水对照组。黄芪对CVB3-RNA的复制有良好的抑制作用,其机制与β-干扰素(β-IFN)无关。
Using Balb/c mice infected with Coxsackie virus B3(CVB3)as a model,the effect of Astragalus membranaceus(AM)on CVB3-RNA has been observed in myocardial tissues of mice by RNA-RNA in situ hybridization with negative-strand RNA probes leballing with 35S and quantitative imaging analysis of positive hybridization signals.The mechanism of its effect on CVB3-RNA has also been investigated by induction with AM and detection of beta-interferon(β-IFN).Results showed that the copy numbers of CVB3-RNA as well as the histologic necrotic sizes in myocardial tissues of AM treated infected mice were significantly smaller than that in infected normal saline treated mice(P<0 .01,P<0.05) respectively,suggesting that AM could inhibit the replication of CVB3-RNA,but its effect on CVB3-RNA was not correlated with induction of β-IFN.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1994年第11期664-666,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
上海市科委资助
关键词
柯萨奇B3病毒
CVB3
病毒性心肌炎
黄芪
RNA-RNA in situ hybridization, Coxsackie B_3 Virus ribonucleic acid,β-interferon,myocarditis,Astragalus membranaceus