莪术对硫酸镍诱导的人外周血淋巴细胞非程序脱氧核糖核酸合成的影响

Effects of Rhizoma Zedoariae on Nickel Sulfide Induced Unscheduled DNA Synthesis of Human Lymphocytes

以人外周血淋巴细胞非程序ＤＮＡ合成（ＵＤＳ）为观察指标，研究了莪术和硫酸镍的遗传毒性效应及莪术对硫酸镍诱导的人外周血淋巴细胞ＵＤＳ的影响。结果表明，莪术对人外周血淋巴细胞ＵＤＳ无诱导效应，但当剂量达到１ｇ／ｍｌ时，可显著阻抑３Ｈ－ＴｄＲ向细胞ＤＮＡ的掺入。硫酸镍可大量诱发人外周血淋巴细胞ＵＤＳ，莪术对硫酸镍和紫外线诱导的人外周血淋巴细胞ＵＤＳ有明显抑制，且存在剂量效应关系。提示莪术在一定剂量时可明显减轻硫酸镍对人外周血淋巴细胞ＤＮＡ的损伤，而其本身无致突变作用。但当莪术剂量达到１ｇ／ｍｌ时，对ＤＮＡ的修复合成可造成抑制。

The study was designed to investigate the toxic effects of Rhizoma Zedoariae(RZ) and nickel sulfide,and the effect of RZ on nickel sulfide induced unscheduled DNA synthesis(UDS) of human lymphocytes using an UDS assay of human lymphocytes.The results showed that TZ did not induce UDS,it could inhibit 3H-TdR incorporation with intracellular DNA when its' dose was 1g/ml,that nickel sulfide and ultraviolet could largely induce UDS,that might be inhibited by RZ with a dose dependent relationship.These suggested that nickel sulfide induced damage of DNA might be inhibited by RZ,RZ was not mutagenic and DNA synthesis might be inhibited when the RZ dose amounted to 1g/ml.