补肾中药对雄激素致不孕大鼠肾上腺皮质形态学观察

Effect of Kidney Tonifying Herbs on Morphological Changes of Adrenal Cortex in Androgen-Sterilized Rats

本实验选用ＳＤ雌性大鼠幼仔，出生第９日龄注射丙酸睾丸酮制作雄激素致不孕大鼠，第８０日龄灌服补肾中药水溶液２周，第１００日龄心脏灌注处死。采用光镜、透射电镜、银胶染色法和新生物素－生物复合法，分别测定核仁组成区嗜银蛋白、增殖前期细胞核仁抗原，观察肾上腺皮质的形态学变化。结果显示：在调节性腺轴功能过程中，包括了对肾上腺的调整。补肾中药水溶液能够使增生的肾上腺网状带恢复正常；细胞内增多的脂滴数和核仁组成区嗜银蛋白、增殖前期细胞核仁抗原阳性细胞计数达正常范围。

Young rats of 9-day old with testosterone propionate subcutaneously injected were used as an androgen sterilized rats (ASR) model. On 80th day, herbal extract of tonifying the Kidney was fed for 14 days, all animals were sacrificed by cardiac perfusion method on 100 days of ages. Morphological studies with light and electron microscope, immunohistochemical studies with argyrophilic nucleolar organiser region(AgNOR) and proliferating cell nucleolar antigen(PCNA) were used for observations on the morphological change of adrenal cortex. In control group(treated with testosterone propionate only) the reticular zone of the adrenal cortex extented widely in which cellular fatty drops, AgNOR and PCNA increased apparently. In animal treated with herbs, the width of the reticular zone, the numbers of AgNOR and PCNA reduced significantly to normal levels (P<0.01, P<0. 01, P<0 .01) and numbers of fatty drops also decreased. It suggests that during inducing ovulation in ASR tonifying Kidney herbs simultaneously regulated the adrenal function besides the chief function on pituitary-ovary regulation.