应用PCR技术对输卵管妊娠患者性传播疾病病原体感染的研究

Pathogen analysis of tubal pregnancy by polymerase chain reaction

目的探讨输卵管妊娠(TP)患者性传播疾病(STD)病原体的感染情况。方法2001年1月至2002年12月间,东莞市人民医院及中山大学东华医院,应用聚合酶链反应技术对249例TP患者的宫颈分泌物及子宫内膜活检标本进行了7种常见STD病原体的检测。结果解脲支原体(UU)的检出率最高,达27.71%和23.29%,其次为沙眼衣原体(CT)20.88%和17.27%,与对照组比较差异有显著性(P<0.05),淋球菌(NG)检出率为7.63%和8.84%、乙型肝炎病毒(HBV)检出率6.02%和4.81%、乳头状瘤病毒(HPV)检出率为3.61%和4.02%、单纯疱疹病毒(HSV)检出率为2.41%和1.20%、梅毒螺旋体(TMP)检出率为0.40%和0。同时还发现各种病原体的上行性感染及混合感染情况。结论与TP相关的病原体中以UU最多见,各种病原体混合感染占一定比例。

Objective To explore the states of the pathogens related to sexually transmitted diseases (STD) in tubal pregnancy(TP). Methods Seven common kinds of pathogens of STD were determined in cervical specimens and endometrial biopsies resected in 249 cases tubal pregnancy by PCR technique. Results Of the 249 cases it was found that Ureaplasma urealyticum (UU) was 27.71% and 23.29% , Chlamydia trachomatis (CT) 20.88%and 17.27% , which were the first two kinds of pathogens.While Neisseria gonorrhoeae(NG) was 7.63% and 8.84% , Hepatitis B virus (HBV) 6.02% and 4.81% , Human papillomavirus(HPV) 3.61% and 4.02% , herpes simplex virus (HSV) 2.41% and 1.20% , Treponema pallidum (TMP) 0.40% and 0, respectively. There were significant differences in the positive rates of UU and CT detecting (P<0.01). Meanwhile , some cases were infected with several kinds of pathogens. Conclusion These data indicate that UU was the most common pathogen, and a proportion of mixed types of pathogens can also be found in the patients with TP.