HPLC法测定增智颗粒剂中紫丁香甙的含量

Determination of Syrigin in ZengzhiGranule by HPLC

采用ＺｏｒｂａｘＳｉｌ柱，以氯仿－异辛烷－甲醇－冰醋酸（３０：１３：４：３）为流动相，２７０ｎｍ波长下测定增智颗粒剂中紫丁香甙的含量。结果表明，其加样回收率为１０２．５２±１．９８％，ＲＳＤ为１．９３％，紫丁香甙的含量为０．０５４３±０．０００５ｍｇ／ｇ。

On Zorbax Sil column with a mobile phase of chloroform-isooctane-methyl alcoliol-acetic acid(30:13:4:3),the content of syrigin in Zengzhi granules was determined by HPLC at 270nm。The results show that the recovery is102.52±1.98%and RSD 1.93%。 The content of syrigin is 0.0543± 0.0005mg/g。