摘要
目的介绍一种时间短、花费少的神经干细胞培养方法。方法先用含10%胎牛血清的DMEM培养液预培养神经干细胞48 h,换含表皮生长因子、碱性成纤维细胞生长因子、B27的DMEM/F12培养液继续培养干细胞球。血清诱导分化后行nestin免疫荧光染色。结果血清预培养48 h后即可见神经干细胞聚球,nestin免疫荧光染色为阳性。结论血清预培养可使神经干细胞聚球速度加快,促进神经干细胞增殖。
ObjectiveTo introduce a method of promoting neural stem cells proliferation.MethodsNeural stem cells were cultured with medium DMEM containing 10% fatal bovine serum for 48 h, then changed medium DMEM/F12 containing epidermal growth factor, basic fibroblast growth factor and B27. Immunohistochemistry was used to identify nestin expression.ResultsNeural stem cells got together fast and formed neurospheres within 48 h and expressed nestin. ConclusionPro-culture with serum can promotes neural stem cell proliferation.
出处
《中国康复理论与实践》
CSCD
2005年第5期339-340,共2页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家自然科学基金(No.30370543)
关键词
神经干细胞
预培养
血清
增殖
neural stem cell
pre-culture
serum
proliferation
