大黄对内毒素所致肠源性感染治疗作用的实验研究

Experimental Study on the Effect of Rhubarb on Enterogenous Infection Caused by Endotoxin

本实验旨在证明大黄是否对内毒素所致肠源性感染具有治疗作用.以雄性ICK小鼠腹腔注射内毒素0.4mg,24小时后处死;大黄每鼠每次10mg,分即刻和12小时于胃管喂入.大黄组小鼠细菌移居发生率和各内脏含菌量显著低于内毒素组和安慰剂组(P<0.01);大黄组小鼠各组织SOD含量明显高于内毒素组和安慰剂组(P<0.01),而LPO含量则与之相反(P<O.01);病理学显示大黄明显减轻内毒素所致小肠粘膜损伤.大黄对内毒素所致肠源性感染具有明显疗效.

experiments was designed to determine whether Chinese traditional medicine Rhubarb could have a good curative effect on enterogenous infections caused by endotoxin. Male ICR mice received intraperitoneal injection of endotoxin at a dose of 0. 4mg per mouse, and were killed 24 hours later. Rhubarb (10mg each time) was administrated to the mice via stomach tube immediately and 12 hours after injection. The results revealed that the incidence of bacterial translocation and the number of viable bacteria contained in the liver, spleen and mesocolon of the mice in the rhubarb group were much lower than that in the endotoxin group and that in the placebo group (p<0. 01 ); whereas the mice in the rhubarb group had a greater quantity of SOD and a lower quantity of LPO in serum,the liver and the small intestinal tissues than did the mice in endotoxin group and that in placebo group (p<0. 01);and microscopic examination showed the degree of histologic damage in the jejunum of the mice in the rhubarb group was less than those in the endotoxin group or placebo group. All these results suggested how and why rhubarb had an obvious curative effect on enterogenous infections induced by endotoxin.