HPLC法测定复方首乌补液中二苯乙烯苷的含量

Determination of 2, 3 , 5 , 4′-tetrahydroxystilbene-2-O-β-D-glucoside in Fufang Shouwu Buye by HPLC

目的:建立测定复方首乌补液中二苯乙烯苷(2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷)含量的高效液相色谱法。方法:hypersil ODS柱,流动相为乙腈:水(30:70);流速1.0mL/min,检测波长320nm。结果:2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷线性范围为25-400mg/mL,r=0.999 5;平均回收率为99.4%。结论:本方法分离效果好,方法简便、快速,适用于该制剂的含量测定。

Objiective: To establish a HPLC method for determination of 2 ,3 ,5 ,4′-tetrahydroxystilbene-2-O-β-D-glucoside in Fu fang Shouwu Buye:. Method : A hypersil ODS column with a mobile phase composed of acetonitrile-water(30:70) was used. The detection wavelength was 320nm. Results: The linear response range of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside was 25-400mg/mL( r = 0. 999 5). The mean recovery was 99. 4%. Conclusion: The method is simple,rapid and accurate.