上皮性卵巢癌P53蛋白及增殖细胞核抗原的表达及意义

P53 protein and proliferating cell nuclear antigen expression in epithelial ovarian carcinoma

目的探讨P53蛋白和增殖细胞核抗原(PCNA)表达与上皮性卵巢癌组织学分级及预后的关系。方法62例上皮性卵巢癌同时用免疫组织化学方法检测P53蛋白和PCNA表达。结果在高分化、中分化、低分化卵巢癌中P53蛋白阳性率分别为32%(10/31)、65%(8/20)、73%(8/11);PCNA阳性率分别为45%(14/31)、65%(13/20)、91%(10/11),在三组间P53蛋白、PCNA的阳性率及表达强度差异均有统计学意义(P<0.05)。结论P53蛋白和PCNA在卵巢癌的诊断、组织学分级中具有重要价值;P53蛋白和PCNA的表达可以作为判断卵巢癌预后的不良指标。

Objective To access the histopathologic grade and the prognostic value of the expression of both P53 protein and proliferating cell nuclear antigen (PCNA) for the epithelial ovarian carcinoma.Method Sixty two cases of epithelial carcinoma were retrospectively reviewd.P53 and PCNA expressions were detected by immunohistochemical staining.Result In well differentiated,moderately differentiated,and poorly differentiated ovarian carcinomas,the frequency of P53 protein positivity was 32% (10/31),65%(8/20),and 73%(8/11) respectively;the frequency of PCNA positivity was 45%(14/31),65%(13/20),and 91%(10/11).The P53 protein,PCNA positivity and expression strength showed a significant statistical difference among the three differentiations (P<0.05).Conclusion The expression of P53 protein and PCNA has great values in the histopathologic grading and prognosis of ovarian carcinoma.P53 protein,PCNA expression can be used as a marker for an unfavorable prognosis on ovarian carcinoma.