聚合酶链反应在诊断结核性胸腔积液中的应用

Polymerase chain reaction for the diagnosis of tuber-culous pleural effusion

应用聚合酶链反应（ＰＣＲ）技术对６２例胸水进行结核菌ＤＮＡ检则，并同时与胸水涂片及培养结果进行比较。结果显示：３４例结核性胸水涂片抗酸染色、结核菌培养和ｐＣＲ检测阳性率分别为５．９％、８．８％和５２．９％，后者显著高于前二者（Ｐ均＜０．０１）。２８例非结核性胸水涂片和培养结果均呈阴性，但ＰＣＲ有４例（１４．３％）出现阳性。结果表明ＰＣＲ直接检测胸水中结核菌显示出快速、敏感和高效等优点。同时还对影响ＰＣＲ检测结核菌的某些因素作了分析。

olymerase chain reaction was applied to detectDNA of mycobacteriunl tuberculosis in 62 patientswith pleural effusion , and result of PCR was comparedwith those of conventional procedures. It was foundthat in 34 tuberculous pleural effusion, the positiverates of pleural effusion acid-fast staining, culture andPCR was 5. 9% , 8. 8% and 52. 9% , respectively. Thesensitivity of PCR was much higher than those of acidfast staining and culture (all P<O. 01 ). The results ofacidfast staining and culture in 28 nontuberculous effusion were negtive, there were, however, 4 PCRpositive results within this group (14. 3%). Our re-sults suggested that for the diagnosis of mycobacterium tuberculosis PCR is a rapid, sensitive and specificprocedure. Some factors that affected the results ofPCR were discussed.