丹参对博莱霉素所致大鼠肺纤维化保护作用的实验研究

Study of Salvia miltiorrhiza on bleomycin-induced pulmonary fibrosis in mice

目的:探讨丹参对博莱霉素所致大鼠肺纤维化的保护作用及其作用机理。方法:通过气管内注射博莱霉素复制大鼠肺纤维化模型,在造模后的d1和d7开始给予丹参注射液治疗,分别在d7、d28处死大鼠,对肺组织匀浆中的脂质过氧化物(MDA)、谷胱甘肽(GSH)、羟脯氨酸(HYP)和肺组织病理学变化测量。结果:在造模后d1给予丹参注射液治疗能抑制实验性肺纤维化大鼠的肺组织匀浆中的MDA、HYP异常升高和GSH的下降,减轻其肺部的病理损害;在造模后d7给予丹参注射液治疗,丹参治疗组与模型组上述观察指标比较无统计学差异。结论:丹参注射液可预防大鼠肺纤维化,对已形成的肺纤维化无逆转作用。

AIM: To study protective effects of Salvia miltiorrhiza on bleomycin (BLM)-induced pulmonary fibrosis in mice. METHODS: Animal model with pulmonary fibrosis in SD mice was replicated by intratracheal injections of bleomycin and Salvia miltiorrhiza injection were given after replicated 7th day. Rats were executed in 7th and 28th day, MDA, GSH and HYP in lung tissue bomogenate and the lung histopathological change were detected to investigate the effects of Salvia miltiorrhiza injection on lung fibrisis form in different stages. RESULTS: Salviae miltiorrhizae injection given after replicated first day restrained the increase of MDA and HYP, and decreased the GSH of lung tissue bomogenate, and lighten pathological lesion. Salvia miltiorrhiza injection given after replicated the 7th day had no statistical difference between Salvia miltiorrhiza therapy groups and model group. CONCLUSION: Salvia miltiorrhiza injection can prevent mice pulmonary fibrosis from and has no side effects on formative rat pulmonary fibrosis.