摘要
建立银黄片中黄芩苷含量测定方法。采用高效液相色谱法,色谱柱:迪马C18色谱柱,甲醇-水-冰醋酸(50∶50∶1)为流动相,检测波长274nm。黄芩苷在0.1008～1.008μg之间线性关系良好,r=0.9998,回收率为97.2%,RSD为1.2%。该方法操作简单,分离效果好,灵敏度高,可作为银黄片的质量控制标准。
A HPLC method for the determination of baicalin in Yinhuang tablets was established. A Diamonsil C_ 18 column was used with methanol-water- glacial acetic acid (50∶50∶1) as mobile phase. The detecting wavelength was 274 nm. The linear range was 0.1008~1.008 μg (γ = 0.9999) and average recovery was 97.2%, RSD= 1.2%. The method was simple and sensitive with good separation efficiency. It could be used in the quality control of Yinhuang tablets.
出处
《中国药事》
CAS
2005年第6期345-346,共2页
Chinese Pharmaceutical Affairs
