外源诱导提高茶树EGCG含量过程的蛋白质组差异分析(英文)

Differential Proteomic Analysis in Normal Tea Shoot and Exogenous Induced Tea Shoot for Increasing EGCG Content

通过无公害化学诱导子的诱导可使茶树芽叶的EGCG含量提高20.15%～25.00%。为了探明诱导的分子机制,用固相pH梯度双向凝胶电泳分离诱导芽叶与正常芽叶的总蛋白质,结果获得了分辨率和重复性较好的双向电泳图谱,差异表达分析发现,诱导出现的特异蛋白有14种,诱导消失的特异蛋白有8种,诱导表达上调相差10倍的特异蛋白有11种,诱导表达下调相差10倍的特异蛋白有6种。选取两个差异蛋白质点进行基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)测定其酶解后的肽质指纹图谱,通过http:/www.matrixscience.com网站,利用Mascot软件检索NCBInr数据库。查询结果:一种为光合系统Ⅰ的铁硫蛋白,另一种为未知蛋白。这些结果表明,茶树诱导芽叶与正常芽叶的蛋白质组存在差异,这些特异蛋白可能在诱导过程中起着重要的作用。

Induced by harmless inducer, the EGCG content of tea shoots improved 20.15%~25.00%. To probe into the molecular mechanism, the immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis was used to separate the total proteins of induced tea shoots and normal tea shoots. The two- dimensional polyacrylamide gel electrophoresis map with high resolution and repeatability was produced. Differential expression analysis indicated that 14 specific proteins were emerged in induced shoot and 8 disappeared, 11 expressed above ten-fold, and 6 below one-tenth. Two differential protein spots were selected for MALDI-TOF-MS to determine the post-enzymolysis peptide mass fingerprinting. Using Mascot to search NCBInr database on website at http://www.matrixscience.com. The results were as follows: one was photosystem I iron-sulfur protein, and the another unkown. The results showed that there were proteome difference between induced and normal tea shoots, that the specific protein might play an important part during inducing process.