摘要
为了建立β2微球蛋白(β2-m)时间分辨荧光免疫分析。以羊抗兔抗体包被板条,双功能螯合剂异氰酸苄基二乙烯三胺五乙酸络合Eu3+及标记β2-m,发光增强系统为以β二酮体为主的增强液。采用竞争法建立β2-m时间分辨荧光免疫分析,数据采用双对数法函数和四参数logistic函数数据处理程序处理。结果此法的批内和批间CV分别为2.25%和2.91%,平均回收率为101.06%,灵敏度为0.06mg/L,可测范围为0.06～12mg/L。本方法与白蛋白、肌红蛋白均无交叉反应。溶血对本法无影响。临床初步应用所检测结果与放免法吻合。试验结果表明,β2-m-TRFIA法的敏感度、特异性、准确度等指标符合临床诊断要求。
The two,site time,resolved fluoroimmunoassay (TRFIA) was established in this study for the detection of β2,microglobulin (β2,m). The tested specimen or β2,m reference standard was added to the micro,titer plates coated with sheep anti rabbit IgG antibody, and diluted rabbit anti,β2,m antibody and Eu3+,β2,m reaction solution were added. After incubation at 25℃ for 1.5 hours and washing for 4 times, the enhancement solution was then added. The fluorescence testing was performed by AutoDELFIA,1235. The intra,and inter,assay CV of this TRFIA were 2.25% and 2.91% respectively. The recovery rate was 101.06% and the sensitivity was 0.06 mg/L, with a range of testing from 0.06 mg/L to 12 mg/L. There was no cross reaction with albumin and myohemoglobin, and hemolysis showed no any influence on this method. The result of preliminary application was consistent with those of radioimmunoassay.
出处
《现代免疫学》
CAS
CSCD
北大核心
2005年第1期53-55,共3页
Current Immunology
