持续高氧对新生大鼠肺组织胶原的动态影响

Dynamic effects of inhaling high concentration of oxygen on the expression of collagen in lung of neonatal rats

目的探讨持续高氧对新生大鼠肺组织胶原的影响。方法足月新生大鼠分别持续吸入90%以上的高氧和空气,于3、7、14、21d,通过染色、化学法和免疫组化,动态比较肺组织中胶原相对含量、羟脯氨酸含量以及I型胶原蛋白的分布和半定量变化。结果(1)7d时肺间质蓝色胶原所占的面积百分比较对照组增加(P<0.05),14d和21d更为明显(P<0.01);(2)7d起高氧组羟脯氨酸含量高于空气组(P<0.05),在14d(P<0.05)和21d(P<0.01)持续高于对照组。(3)7d时高氧组较空气组胶原在肺间质中的表达增加(P<0.05),14d(P<0.05)和21d(P<0.01)也明显高于对照组。结论持续吸入高氧可致胶原在新生大鼠肺组织间质中的沉积增加。

Objective To investigate effects of inhaling high concentration of oxygen on the collagen in lung of neonatal rats.Methods Full-term newborn rats were continuosuly exposed to more than 90% oxygen or room air (21% O_2) and the rats were killed at 3、7、14、21 d respectively.We used Masson staining to stain lung collagen,chemical methods to measure the content of hydroxyproline,and immunohistochemistry to test the expression of type I collagen in the lung.Results (1)In hyperoxia group,the percentage of blue collagen positive area increased at 7 d(P<0.05),and kept increasing at 14 d and 21 d (P<0.01).(2)The content of hydroxyproline in hyperoxia group was higher than that of the control group since the 7th day.(3)The expression of type I collagen in the lung interstitium of hyperxia rats began to increase at 7 d,and were significantly higher than that of control group at 14 d and 21 d.Conclusion After continuously inhaling high concentration of oxygen,collagen were increased in the lung interstitium of neonatal rats.