阿魏酸钠对CCL4致肝硬化大鼠肝缺血再灌注损伤的影响

Protective Effect of Sodium Ferulate on Liver Ischemia/Reperfusion Injury in Rats with Cirrhosis

目的:研究阿魏酸钠(SF)对肝硬化大鼠肝脏缺血再灌注(I/R)损伤的保护作用。方法:用50%四氯化碳油溶液皮下注射的方法制作肝硬化大鼠模型,将30只肝硬化大鼠随机均分为3组。A组:假手术组(10只);B组:对照组(10只);C组:SF保护组(10只)。B、C组分别从尾静脉缓慢注入生理盐水1.5mL、SF1.5mL(150mg/kg)后,完全阻断大鼠肝门血流30min,比较各组肝脏再灌注2h后的肝功能,肝组织抗氧化能力、一氧化氮(NO)含量及肝脏形态学改变。结果:肝脏再灌注2h时,与对照组比较,SF保护组大鼠肝组织丙二醛(MDA)含量显著减少(P<0.01),超氧化物歧化酶(SOD)和NO含量显著增高(P<0.01),血清丙氨酸氨基转移酶(ALT)、天门冬酸氨基转移酶(AST)、乳酸脱氢酶(LDH)活性显著降低(P<0.01),对肝脏显微结构和超微结构损伤较轻。结论:SF对肝硬化大鼠肝I/R损伤有明显的保护作用。

Objective To study the preventive effect of the damage of ischemia-reperfusion(I/R) in rat liver with cirrhosis by applying sodiuim ferulate (SF) Methods The model of liver cirrhosis was established by subcutaneous injection of carbon tetrachloride(CCL4) in rats.Then 30 rats of liver cirrhosis were randomized into 3 groups.In group A(n=10),sham operation was performed.In control group B and protection group C with 10 rats in each,were injected NS 1.5 ml or SF 1.5 ml(150 mg/kg) by tail vein,respectively,then hepatic warm ischemia was achieved for 30 min.The liver function parameters superoxide dismutase(SOD),malonyldialdehyde(MDA),nitric oxide(NO) in liver tissues,and the liver histologic changes after 2 hours of reperfusion in both groups were compared.Results After 2 hours of reperfusion,compared with controlled group,the serum AST,ALT,LDH and MDA contents significantly decreased(P<0.01);the liver contents of SOD and NO significantly increased(P<0.01) in SF protection group;light and electron microscopy showed that in SF protection group the degree of liver injury was much less than in control group. Conclusion SF plays a protective effect on I/R injury of cirrhotic liver in rats.