肝脂素对急性白血病骨髓基质细胞造血调控作用的研究

Regulatory effects of hematopoiesis of acute leukemia bone marrow stromal cells after treated with YS2H

目的研究肝脂素对急性白血病骨髓基质细胞造血的调控作用。方法ELISA法检测细胞因子,光镜观察骨髓基质细胞形态。结果肝脂素可选择性作用于急性白血病骨髓基质细胞,使网状细胞转变为小梭形正常基质细胞。抑制急性白血病基质细胞集落的生长,使ALL与AML基质细胞集落生长情况与正常趋向一致,ALL与AML的TNFα分泌明显下降(P<0.05),AML的IL-6明显下降(P<0.01),IL-1升高。结论肝脂素能诱导急性白血病造血微环境中的基质细胞向正常转化,发挥正常造血调控作用,为急性白血病的脂类治疗提供了一种新的方法。

Objective To study the regulatory effects of hematopoiesis of acute leukemia bone marrow stromal cells after treated with YS2H. Methods Cytokines were examined by ELISA methods; Bone marrow stromal cells conformation were observed by light microscope. Results YS2H could act on bone marrow stromal cells selectively and make reticulum cell changing to normal stromal cells, inhibit the growth of colony of acute leukemia stromal cells by making ALL and AML stromal cells correspond with normal cells in growth trend and in secretion level of TNFa, making IL-6 decrease and IL-1 increase. Conclusion YS2H could induce stromal cells in acute leukemia microenvironment into normal cells and play a role in the normal regulation of hematopoiesis.