针灸对小鼠骨髓细胞染色体畸变率、姊妹染色单体交换率的影响

Effects of acupuncture and moxibustion on chromosome aberrations rate and sister chromatid exchange rate in bone cells of mice

目的:研究针灸的抗突变作用。方法:以小鼠为实验动物,以环磷酰胺为诱变剂。随机分正常对照组,阳性对照组,针灸预防Ⅰ、Ⅱ组,针灸治疗Ⅰ、Ⅱ组。针灸观察组针刺"足三里",艾灸"关元"穴,检测各组小鼠骨髓细胞染色体畸变率、姊妹染色单体交换率的差异。结果:阳性对照组与正常对照组比较,骨髓细胞染色体畸变率和姊妹染色单体交换率明显升高,而针灸各组与阳性对照组比较明显降低(P<0.01)。结论:针灸可以拮抗由环磷酰胺诱导的小鼠骨髓细胞染色体畸变率和姊妹染色单体交换率的升高。针灸具有抗突变作用。

Objective To study the anti-mutation action of acupuncture and moxibustion. Methods Mice were randomly divided into 6 groups, group 1 (normal control group) , group 2 (positive control group) ,group 3 (prevention group Ⅰ ), group 4 (prevention group Ⅱ ) , group 5 (treatment group Ⅰ )and group 6 (treatment group Ⅱ ). The mice in the group 2-6 were treated by cyclophosphamide (ip, 50 mg/kg body weight), and in the 3-6 groups were given acupuncture at 'Zusanli' (ST 36) and moxibustion at 'Guanyuan' (CV 4). At the end of experiment, all the mice were decapitated and chromosome aberration rate and sister chromatid exchange rate of bone marrow cells were investigated. Results The chromosome aberration rate and the sister chromatid exchange rate of bone marrow cells in the positive control group increased significantly as compared with the normal control group, while they decreased significantly in the group 3, 4, 5, 6 as compared with the positive control group(P<0. 01). Conclusion Acupuncture and moxibustion have anti-mutation action, inhibiting the increase of chromosome aberrations and sister chromatid exchange of bone marrow cells in mice induced by cyclophosphamide.