黑色素瘤抗原基因和脆性组氨酸三联体基因在诊断肺癌淋巴结微转移的应用

Detection of occult micrometastasis using MAGE and FHIT genes in lymph nodes of patients with non-small cell lung cancer

目的检测FHIT和MAGE-1、-2、-3、-4基因mRNA在非小细胞肺癌(NSCLC)淋巴结中的表达情况。方法采用逆转录聚合酶链反应(RTPCR)方法检测53例患者的111组淋巴结脆性组氨酸三联体基因(fragilehistidinetriad,FHIT)和MAGE-1、-2、-3、4mRNA的表达。结果应用RTPCR技术检测MAGE和FHIT基因mRNA表达在NSCLC淋巴结微转移阳性率分别为41.4%(46111)和35.1%(39111),差异无显著性(P>0.05)。非肺癌患者淋巴结四种MAGE和FHIT基因表达均为0。应用常规病理检查和应用RTPCR技术检测MAGE和FHIT基因在淋巴结微转移阳性率分别为27.9%(31111)和44.1%(49111),差异有显著性(P<0.05)。结论MAGE基因是应用RTPCR法检测非小细胞肺癌淋巴结微转移的较好的分子标志物,与FHIT基因联合检测可能有助于早期诊断肺癌淋巴结微转移。

Objective To investigate the expressions of MAGE-1,-2,-3,-4 and fragile histidine triad (FHIT)genes in lymph nodes of p atients with non-small cell lung cancer (NSCLC) at mRNA level.Methods 111 stations of lymph nodes from 53 patients with NSCLC were studied t o detect mRNA for MAGE-1、-2、-3、-4 and FHIT genes by using reverse transcr iption-polymerase chain reaction assay(RT-PCR). Results O f the 111 stations of lymph nodes,at least one of these MAGE genes was expressed 41.4%(46/111) in of the samples,and FHIT genes was expressed 35.1%(39/111).O f the samples of lung and the lymph nodes of the patients without lung cancer,th e MAGE-1,-2,-3,-4 and FHIT were expressed at mRNA level in 0%.The expressio n rate in samples of lymph nodes using RT-PCR (41.4%,46/111)was significantly higher than that using routine histopathological examination (27.9%,31/111). Conclusion Micrometastasis in lymph nodes of patients with NSCLC could be diagnosed by investigated the expressions of MAGE genes at mRNA level .The establishment of FHIT may lead to an earlier diagnosis of metastasis for l ung cancer.