肝细胞癌细胞核DNA流式细胞术分析

Flow Cytometric Analysis of Nuclear DNA in Hepatocellular Carcinoma

本文用流式细胞术(FCM)对44例肝细胞癌(HCC)石蜡包埋组织进行了DNA含量及细胞周期分析.本组HCC的异倍体率为54.5％.异倍体HCC在50岁以下患者的出现率高于50岁以上者,其癌栓形成率显著高于二倍体HCC,预后亦较差.DNA倍性与患者性、AFP水平、临床分期、病理分级及瘤径(3cm为界)等因素无关.HCC增殖活性显著高于正常肝,增殖指数(PI)>0.40者预后较差.PI与AFP水平、病理分级、癌栓形成及瘤径间均未显示相关性,其意义有待进一步研究阐明.

The nuclear DNA content of 44 paraffin-embedded heaptocellular carcinoma(HCC)specimens was measured by flow cytometry. 54. 5% of the HCCs in this group displayed aneuploid DNA content. The incidence of aneu-ploid HCC was higher in patients under 50 years of age. Significant higher rate of vascular tumor embolization and poorer prognosis were also demonstrated in aneuploid HCC. However,there was no significant correlation in between DNA ploidy and patient' s sex,serum AFP titer,clinical status,pathological grade,or tumor size(3cm as threshold). The proliferation index(PI)was much higher in HCC as compared to normal liver,and HCC with PI over 0. 40 showed poorer prognosis. In this study,PI did not show significant correlation with serum AFP titer,pathological grade,vascular tumor embolization,or tumor size,the implications of which need to be clarified yet.