恶性淋巴瘤患者血浆中环磷酰胺的测定及药代动力学研究

Determination of Plasma Concentration and Pharmacokinetics of Cyclophosphamide on lymphoma Patient

本文采用紫外分光光度法测定了１４例用环磷酰胺治疗的恶性淋巴瘤患者体内的血浆药物浓度，并依据ＡＩＣ差别程序按静推二室开放模型拟合后进行非线性迭代回归计算药代动力学参数，结果表明：ＣＴＸ在体内以较快的速度进行分布，经过２４ｈ左右药物几乎全部被消除；主要参数为：ｔ３／２α１３．７９±１１．９０ｍｉｎ，ｔ１／２β３３９．７３±２０４．２７ｍｉｎ，ＣＬ１．４５３×１０＾２ｍｌ／ｍｉｎ，ＡＵＣ（９．

A sensitive colorimeter technic is described for determining cydophosphamide(CTX)in plasma. It isbased on the fact that active alkylating metabolites of CTX became purple in color by absorbing 575nm at basic pH. The minimal amount of detection is 1. 5ug/ml in plasma. Plasma level of CTX in 14 patients with Lymphoma had been mea-sured after i. v. dosage of 600-800mg/m2.of CTX. The concentration-time curve fitted is well with the compartment open model. The concentration-time curve was calulated with non-linear iteration regression by computer. The pharmacokinetic parameters showed that tl/2α, tl/2β,CL, AUC and Vd were 13. 79min, 339. 73min, 1. 453 × 102ml/min, 9. 554 × 104ng/ml min and 0. 89L/kg respectively.