TA_2系小鼠B淋巴细胞型淋巴瘤淋巴道转移过程形态学观察及DNA含量分析

Spontaneous Lymphatic Spread and DNA Content of.Transplantable in B-lymphoma TA2 Mice

本实验利用我室建立的TA_2.系小鼠可移植性B淋巴细胞型恶性淋巴瘤株.第43代瘤组织,移植于动物鼠蹊部皮下,2天后开始增殖形成瘤细胞团块.9天后在局部淋巴结边缘窦内定居繁殖形成转移灶,16天远处淋巴结内查见癌细胞.在实验晚期累及肝、肾组织.淋巴结早期反应为副皮质明显扩大,免疫母细胞数显著增加.实验晚期生发中心扩大融合,髓索内浆细胞显著增加.应用显微分光光度计检测,发现局部移植瘤的瘤细胞DNA含量及核面积均随时间和转移途径的延续而增大.我们认为DNA含量发生改变是恶性淋巴瘤易转移的重要标志.因此是一个较为理想的自发淋巴道转移实验模型.

An experimental model of transplantable B-lymphoma in TA2 mice was established. The43rd generation tumor tissue was implanted subcutaneously in mice. In two days after inoculation tumor cell began to proliferate and formed nests. Nine days after transplantation,tumor cell were identified in the regional lymph.nodes. At 16th day,tumor cells were found in the remote lymph nodes. In the advanced stage,metastasis were found in liver and kidney. The early responses in the lymph nodes were proliferation of im-munoblasts and expansion of paracortical areas. In later stage of tumor growth ,the mode of reaction in lymph nodes was characterized by the enlargement, fusion of germinal centers and increased number of plasma cell in the medullary cords. Micropectrophotometer study revealed that nuclear area of the implanted tumor cells enlarged in size. It is conjectured DNA content is an important sign of lymphoma metastatic propensity.