宫颈癌变过程中细胞RNA含量的定量分析

Assay of RNA Content in Normal Cells and Cells of Mild,Moderate,Severe Dysplasia and Cancer of the Cervix Uteri

应用显微分光光度计及扫描方法对宫颈正常组织,轻、中、重度非典型性增生,Ⅰ、Ⅱ、Ⅲ期癌组织细胞进行RNA定量分析.结果表明:组织细胞的RNA含量随病变的发展而逐步增大,组织病变越严重,RNA含量越高.提示,对宫颈病变组织的组织化学检测除DNA参数外,增加RNA参数有助于对宫颈癌的鉴别诊断.

RNA content in normal uterus cervical cells as well as cells of mild,moderate and severe dysplastic changes and stage Ⅰ,Ⅱ,Ⅲ cancers of the cervix uteri were measured with micro-fluorometric and microscanning technics. It was shown that RNA content in cells increased with the progress of degree of dysplastic change. The hignest RNA content was observed in stage 1 carcinoma. These findings indicate that besides DNA content,RNA content in cervix cells may be another parameter in the differential diagnosis of cervix tumors.