酶联免疫法测定人乳腺肿瘤及其体液p21蛋白

The Determination of H-ras p21 Protein in Breast Tissue and Serum of Breast Cancer Patient by ELISA

本文研究了乳腺癌组织提取物和病人血清中ras癌基因产物p21蛋白的表达.结果表明良性乳腺瘤与恶性乳腺癌之间有明显差异.使用双抗体夹心法测定组织提取物和体液p21蛋白浓度是有效的.乳腺癌病人体液中p21蛋白含量较低,并且与健康人差异较小.

It is our purpose to study the expression of ras-oncogene product p21 in extract of breast carcinomas and body fluid of the patients. The results show that there is significant difference between the carcinomas and benign tissues. And it seems that the sandwich EIA is effective in determining the content of the tissue extract and the body fluid. The concentration of p21 in body fluid of breast carcinoma patients is too low to be the tumor marker of breast carcinoma for clinical diagnosis by ELISA.