使用极限稀释液体微量培养法检测小儿急性白血病细胞的药物敏感性

Determination of Chemosensitivity in Children's Acute Leukemia by Limiting-dilution Micro-liquid Culture Assay

使用CFU-AML和CFU-ALL两种培养体系对35例小儿急性白血病(AL)进行了祖细胞培养,研究了集落细胞的遗传学和细胞表面免疫标记,发现集落细胞的染色体核型和表面免疫标记均与培养前白血病细胞一致.因此本文把这两种培养体系应用于体外药敏试验中,对部分患儿进行了VP、VLDP、COAP、HOAP四组化疗方案的药敏试验,使用的是极限稀释液体微量培养法.结果表明体外药敏与体内疗效的符合率为11/12.本法具有简单、敏感、客观、无放射性污染等特点,可望为白血病的临床治疗和研究提供帮助.

Thirty-five patients with acute leukemia were studied as to the cellular morphology, karyotype and surface immunological markers of colonies. There were 19 patient with AML with CFU-AML and 16 patients ALL with CPU-ALL. The results showed that the colonies developed in vitro consisted of blast cells which had the same karyotype and surface markers as the original marrow specimens for diagnostic purpose. Using limiting-dilution micro-liquid culture assay, we determined the chemosensitivity of four agents in the combined chemotherapy schemes (VP,VLDP,COAP,HOAP) on patients. The concordance rate was 11/12 between chemosensitivity in vitro and efficacy in vivo.We consider the assay will be valuable in determining the effects of anti-tumor drugs on leukemic progenitor cells. Not only does the assay have simple, sensitive, objective advantages but also the process of culture is devoid of contamination by radioactive elements.