疏肝活血健脾方对PHG大鼠胃组织ET水平影响及防治作用研究

Experimental Studies of Shuganhuoxuejianpifang on Effect on the Endothelin (ET) in the Gastric Tissues of Portal Hypertension Gastropathy (PHG) Rats and the Action of Prevention and Cure

目的:运用一期门静脉缩窄法制备PHG动物模型,从胃组织ET水平,研究疏肝活血健脾方对PHG的防治作用。方法:选择健康雄性wistar大鼠120只,随机分为A、B、C、D、E、F、G、H8组。采用一期门静脉缩窄法制备门脉高压性胃病动物模型。按要求造模灌胃后,在预定时间采胃组织,用免疫组织化学技术图像分析法检测胃底组织ET水平。结果:①A组大鼠胃底组织ET免疫组化平均灰度值较F组升高,两组比较有非常显著性差异(P<0.01);②B组、C组、D组大鼠胃底组织ET免疫组化平均灰度值较E组升高,组间比较有非常显著性差异(P<0.01,B组疗效优于D组(P<0.05)。结论:①ET在PHG的发病机理中起着重要作用。②疏肝活血健脾方对PHG的防治效果肯定。

Objective:Using the method of partial portal vei n ligation (PVL) to develop the experimental model of Portal hypertensive gastropa thy(PHG), from content of ET, to study the principle of PHG and the prevention a nd cure effect of the prescription of smoothing the liver-Qi, acting blood and strengthening the spleen.Methods:120Wistar male rats were divided into eight gro ups randomly- Using the method of partial portal vein ligation (PVL) to develop the experimental model of Portal hypertensive gastropathy(PHG).The rats of ever y groups were got the stomach tissues to test Endothelin (ET) and observed with pathology. Results: The average B-mode titre of ET of group A in gastric fund us tissue are significantly higher than group F using immunohistochemical method (P<0.01). The average B-mode titre of ET of group B、C、D in gastric fundus tissue are significantly higher than group E using immunohistochemical m ethod(P<0.01) The effect of group B is more excellent than group D (P< 0.05). There are many pathologic changes in stomach tissues ,such as arterio -venous shunt formation, communicating branches increasing, microvessel expandi ng and distorted with group A、B、C、D、E、F. But the severity of stomach mucous membrane damages in group E and F were more obvious than other groups, the seve rity of stomach mucous membrane damages in group B is the most light.Conclusion: ET has an close relation with the principle of PHG. The prescription of smoothin g the liver-Qi, acting blood and strengthening the spleen has positive effects in preventing and curing PHG.