穴位刺血对变应性鼻炎大鼠下丘脑-垂体-肾上腺轴ACTH的影响

Effects of pricking blood therapy on ACTH contents in the hypothalamus-pituitary-adrenal axis in the rat of experimental allergic rhinitis

目的:探讨穴位刺血疗法治疗变应性鼻炎的机理。方法:以卵清蛋白建立大鼠实验性变应性鼻炎模型,用放射免疫分析法检测穴位刺血治疗前后鼻炎大鼠下丘脑、垂体、肾上腺、血浆促肾上腺皮质激素(ACTH)含量,并设立中药穴位敷贴、二丙酸氯地米松喷雾治疗对照和正常健康对照组。结果:变应性鼻炎大鼠下丘脑、垂体、肾上腺、血浆ACTH水平明显低于正常对照组(P<0.01)。穴位刺血治疗后上述部位的ACTH水平明显上升,治疗前后比较差异有非常显著性意义(P<0.01);与同期模型组比较差异亦有非常显著性意义(P<0.01);与穴位敷贴及药物治疗对照组比较结果相似(P>0.05)。结论:穴位刺血疗法对实验性变应性鼻炎大鼠下丘脑-垂体-肾上腺轴ACTH有调整作用。

Objective To explore the mechanism of pricking blood therapy (PBT) for allergic rhinitis (AR). Methods The model rat of experimental allergic rhinitis (EAR) was established by administration of ovoglobulin and they were divided into five groups randomly: a model group, a PBT group, a TCM point application group, a beclomethasone dipropionate (BDP) treatment group, and a health control group. Adrenocorticotrophic hormone (ACTH) contents in hypothalamus, pituitary gland, adrenal gland and plasma were determined by radioimmunoas-say before and after treatment. Results The ACTH levels in the hypothalamus, pituitary gland, adrenal gland and plasma in the rat of EAR were lower than those in the health control group (P<0. 01). After treatment of pricking blood ther, ACTH levels in the above tissues increased significantly (P<0. 01) , with very significant differences (P<0. 01) as compared with the model group, similar to those in the BDP group (P>0. 05). Conclusion Pricking blood therapy has a regulatory action on ACTH in HPA axis of rats with experimental allergic rhinitis.