电针对谷氨酸钠诱导的肥胖大鼠脂代谢的影响

Effect of electroacupuncture on metabolism of lipids in rats of obesity induced by sodium glutamate

目的:探讨电针治疗肥胖的机理。方法:大鼠皮下注射15%谷氨酸钠溶液,制成肥胖模型,随机分成空白对照组、模型组、电针组、西布曲明组;实验结束后分别测定各组大鼠血胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)含量,脂蛋白脂酶(LPL)活性及血清瘦素(leptin)和胰岛素(insulin)水平。结果:电针组大鼠体重和Lee’s指数较模型组均明显下降(P<0.01),电针组大鼠TG、TC和LDL-C含量均低于模型组(P<0.01),并且电针组优于西布曲明组(P<0.05);电针组和西布曲明组HDL-C的含量都高于模型组(P<0.01),但两组间没有明显差异;电针组LPL活性较模型组明显升高(P<0.01)。电针组和西布曲明组血清瘦素、胰岛素水平均低于模型组(P<0.05,P<0.01),电针与西布曲明对胰岛素的影响差异没有显著意义,而对瘦素的影响电针组降低的程度比西布曲明组高(P<0.01)。结论:电针刺激能够改善肥胖大鼠的高血脂状态,提高脂蛋白脂酶活性;同时调节血清高瘦素和高胰岛素水平。

Objective To explore mechanisms of acupuncture for reducing of body weight. Methods The rat obesity model was developed by subcutaneous injection of sodium glutamate solution, and the experimental rats were divided into a blank control group, a model group, an electroacupuncture group and a sibutramine group( blood TC, TG, HDL-C, LDL-C contents and lipoprotein lipase (LPL) activity , and serum leptin and insulin levels were determined. Results The body weight and Lee's index, and TG, TC and LDL-C contents in the electroacupuncture group decreased significantly as compared with those in the model group (all P<0. 01), the actions in the electroacupuncture group being superior to those in the sibutramine group; the HDL-C contents in both the electroacupuncture and the sibutramine groups were higher than that of the model group (P<0. 01) with no significant difference between the electroacupuncture group and the sibutramine group; LPL activity in the electroacupuncture group increased significantly as compared with that in the model group (P<0. 01); serum leptin and insulin levels in both the electroacupuncture group and the sibutramine group were lower than that in the model group (P<0.05, P< 0. 01), with no significant difference between the electroacupuncture group and the sibutramine group in the effect on insulin content, and the decrease of leptin level in the electroacupuncture group was more as compared with the sibutramine group (P<0. 01). Conclusion Electroacupuncture can improve the status of high blood lipids, increase lipoprotein lipase activity, and regulate serum leptin and insulin levels in the obestic rat.