隔药灸治疗大鼠溃疡性结肠炎差异表达基因研究

Study on differentially expressed genes of ulcerative colitis in the rat treated by herbs-partitioned moxibustion

目的:探讨隔药灸治疗溃疡性结肠炎的作用机理。方法:将溃疡性结肠炎大鼠随机分为模型组和隔药灸组,并设正常对照组。隔药灸组选取“天枢”,隔药灸治疗大鼠溃疡性结肠炎。应用BiostarR-40s基因芯片进行结肠组织差异表达基因检测,并应用实时荧光定量PCR方法对差异表达基因白细胞介素-1βmRNA(IL-1βmRNA)进行鉴定。结果:筛选出在溃疡性结肠炎大鼠结肠中异常表达、隔药灸治疗后得到调节的差异表达基因174条,其中28条(含已知基因7条)在溃疡性结肠炎大鼠中表达下调的基因经隔药灸治疗后表达升高,146条(已知基因42条)上调基因经隔药灸治疗后表达降低。结论:大鼠溃疡性结肠炎的发生涉及多种基因表达异常,隔药灸可通过调节IL-1β等诸多基因的表达,起到治疗作用。

Objective To probe the mechanism of herbs-partitioned moxibustion for treatment of ulcerative colitis. Methods The rats with ulcerative colitis were randomly divided into 3 groups, normal control group, model group, and herbs-partitioned moxibustion group. The rats in the herbs-partitioned moxibustion group were treated by herbs-partitioned moxibustion at bilateral Tianshu (ST 25). BiostarR-40s gene chip was applied to detect the differentially expressed gene of their colonic tissues and fluorescence quantitative polymerase chain reaction was employed to confirm the results of the microarray analysis with interleukin-1 beta messenger RNA (IL-1β mRNA) expression. Results Of 174 differential expression genes identified, 28 genes (including 7 known genes) elevated in rats of ulcerative colitis were down-regulated and 146 genes (including 42 known genes) reduced in the rat of ulcerative colitis were up-regulated after herbs-partitioned moxibustion treatment. Conclusion Many abnormally expressed genes are involved in occurrence of ulcerative colitis and herbs-partitioned moxibustion can regulate expression of IL-1β and other genes to exert therapeutic effect.