血管性痴呆小鼠海马胆碱乙酰转移酶mRNA变化特征及喜得镇的影响

The effect of hydergine on choline acetyltransferase in neuron of hippocampus in mice with vascular dementia in situ hybridization

目的:观测喜得镇对血管性痴呆小鼠海马神经元胆碱乙酰转移酶mRNA变化的影响,以探讨胆碱乙酰转移酶mRNA变化在血管性痴呆发病中的作用及喜得镇对此变化的机制。方法:双侧颈总动脉线结.反复缺血-再灌注法制备模型,药物组用喜得镇溶液灌胃,利用跳台试验和水迷宫试验观测其行为学改变,采用原位杂交技术观测小鼠海马神经元胆碱乙酰转移酶mRNA的表达变化。结果:喜得镇组小鼠学习、记忆成绩优于模型组(P<0.01),其海马胆碱乙酰转移酶mRNA表达也明显增高(P<0.01)。结论:喜得镇改善血管性痴呆小鼠学习、记忆成绩与其恢复海马低水平的胆碱乙酰转移酶mRNA有关。

Objective:To observe the effect of hydergine on choline acetyltransferase mRNA in neuron of hippocampus in mice with vascular dementia. Methods: The mice were subjected for reperfusion repeatedly on bilateral common carotid arteries by knots to establish the VD models. The behavioral abnormalities were investigated by step-down test and water maze test. Hydergine was given to the mice in medication group, choline acetyltransferase mRNA in neuron of hippocampus were observed in sim hybridization technique. Resuls: Hydergine improved the grades of learning and memory in VD mice. The expression of choline acetyltransferase mRNA in neuron of hippocampus were ameliorated by hydergine. Conclusion: Hydergine improved dysfunction of learning and memory in VD, suggested that hydergine restored expression of choline acetyltransferase mRNA.