人参总皂甙对大鼠脑缺血再灌注后MDA、SOD及细胞凋亡的影响

Effect of ginseng total saponin on MDA, SOD and apoptosis after cerebral ischemic reperfusion in rats

目的:观察人参总皂甙对大鼠脑缺血再灌注的保护作用,探讨其作用机制。方法:将40只大鼠随机分为4 组:假手术组、缺血再灌注组、治疗组1、治疗组2,采用线栓法制备大鼠脑缺血再灌注模型,72h断头取脑,Nissel染色光镜下观察海马CA1区病理形态变化,TUNEL法检测细胞凋亡,同时检测脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)的含量。结果:与缺血再灌注组相比,人参总皂甙治疗组光镜下病理损伤轻,脑组织中MDA含量降低、SOD含量升高,细胞凋亡数降低。结论:人参总皂甙对大鼠脑缺血再灌注损伤具有保护作用,其机制可能与抑制自由基损伤有关。

Objective: To study the protective effect and the functional mechanism of ginseng total sapomin on cerebral ischemia reperfusion injury in rats. Methods: 40 adult Wister rats were randomly divided into 4 groups: sham-operated group, cerebral ischemic reperfusion group, treated group 1 and treated group 2. The model of focal cerebra ischemic reperfusion was made by string-emb. olic method. After 72 hours, the brains were removed to observe the morphological change of the neuron in hippocampus CAl region under optical microscope through Nissel staining, detect apoptosis by TUNEL, detect the content of MDA, SOD in brain tissue. Results: Compared with ischemic reperfusional group, the treated groups had a lighter injury under optical microscopes, a lower apoptosis number, a lower content of MDA, and a higher content of SOD in brain tissue. Conclusion: Ginseng total sapomin provide neuroprotection in cerebral ischemic reperfusion in rats. The mechanism of this effect may be related to inhibiting the injury of free radicals.