脑心通对实验性脑梗死大鼠脑组织IL-6的影响

The effect of Naoxintong on IL-6 expression in the brain tissues of MCAO rats.

目的:探讨脑心通对实验性脑梗死大鼠脑组织中IL一6水平的影响。方法:参照Longa等方法制成大脑中动脉梗死(MCAO)模型,应用干一湿重法观察脑含水量变化、HE染色观察梗死周围炎性细胞浸润、免疫组化方法观察脑组织中lL一6的表达。研究不同剂量脑心通对脑梗死后大鼠行为学评分.脑含水量及脑组织中炎性细胞因子lL一6表达的影响。结果:脑梗死组织周围炎细胞浸润和IL一6阳性细胞表达于梗死后6h开始增多,48h达高峰,并持续到7d。脑心通治疗组大鼠脑梗死后脑水肿减轻,行为学评分降低,脑组织中IL一6阳性细胞减少,大、中剂量脑心通治疗组效果较为显著。结论:脑心通有抑制炎性细胞因子IL_6的过度表达,保护神经元,减轻脑水肿的作用。

Objective: To investigate the effect of Naoxintong on the expression of IL-6 in brain tissues after the middle cerebral artery occlusion (MCAO) in the brain. Methods: The model was established with the suture-occuluded method to observe the effect of different doses of Naoxintong on the behavior changes and brain water content of MCAO rats. HE dyeing and immunohistochemistry staining were used in the study. Results: After MCAO, inflammatory cells began to infiltrate at 6 h in the brain tissues, and reached its peak at 48h. IL-6 expressed increasingly at 6 h, reached maximum at 48h. and continued to 7d. The brain water content, behavior scores of MCAO rats treated with Naoxintong decreased, and to some extent, the revel of IL-6 was inhibited by the use of Naoxintong. The effect of large dose and medium dose of Naoxintong was obviously. Conclusion; Naoxintong can effectively inhibit the expression of inflammatory cell factor IL-6, reduce brain edema, and decrease the behavior disturbance of MCAO rats.