巨噬细胞对A549肺泡细胞癌细胞作用的定量酶细胞化学研究

Enzyme cytochemistry and morphometric study of the effccts of macrophages on A549 pulmonary alveolar Cell Carcinoma cell Iine

为研究巨噬细胞（ＭΦ）抑制、杀伤肿瘤细胞的机理，我们应用酶细胞化学及形态定量技术观察比较正常及活化ＭΦ分别与Ａ５４９肺泡细胞癌细胞作用后，癌细胞内细胞色素氧化酶（ＣＯ），琥珀酸脱氢酶（ＳＤ），乳酸脱氢酶（ＬＤＨ）及酸性磷酸酶（ＡＣＰ）活性的变化。结果见活化ＭΦ（Ｅ）与癌细胞（Ｔ）比值为１０：１时，癌细胞线粒体的ＣＯ，ＳＤ，及ＬＤＨ活性降低，Ｅ／Ｔ＝２０：１时溶酶体的ＡＣＰ活性增加。提示Ｅ／Ｔ适当时，活化ＭΦ可引起癌细胞线粒体及溶酶体的损伤，影响其酶活性引起细胞呼吸和氧化磷酸化等代谢改变，可能是活化ＭΦ抑制、杀伤癌细胞的机理之一。

In order to study the mechanism of the effects of MΦon tumor cells,enzyme cytochemistry and morphometry were used to investigate the activities of cytochrome oxidase(CO),succinate dehydrogenase(SD), lactate dehydrogenase(LDH) and acid phosphatase (ACP) in A549 pulmonary alveolar cell carcinoma cells which had been interacted with normal and CP一activated macrophages respectively.It was found that when E / T = 10:1,the enzyme activity of the cancer cell mitochondria,CO,SD,LDH were decreased,and when E/T=20:1,the activity of the lysosomal enzyme ACP was jncreased.These results indicate that when the E/T ratio was appropriate,activated MΦmay injure the mitochondria and lysosomes and affect the aerobic respira-tion and oxidative phosphorylation of cancer cells.This may be one of the cytostatic and cytotoxic mechanisms of activated MΦon tumor cells