胃癌浸润转移与整合蛋白、IV型胶原酶及细胞外基质的关系

The relation between integrin,type IV collagenase and extracellular matrix in invasion and metastasis of gastric carcinoma

应用免疫组化方法研究整合蛋白ａ＿６亚基、ＩＶ型胶原酶、层粘连蛋白、ＩＶ型胶原及ｒａｓｐ２１在胃癌中的表达。结果表明：ａ＿６亚基与层粘连蛋白在膨胀型胃癌中常呈连续的或断续的线状表达，而在浸润型胃癌中常呈断续斑点小片状表达或甚至几乎消失。提示层粘连蛋白受体和配体的相互作用可影响胃癌的生长方式。ＩＶ型胶原酶在胃癌中表达增强，尤其在浸润性生长和有淋巴结转移的胃癌中更明显，可以作为反映胃癌细胞浸润和转移的标志，且与ｒａｓｐ２１在胃癌中增强表达呈正相关。ＩＶ型胶原的表达与层粘连蛋白的表达常一致，而与ＩＶ型胶原酶的表达呈反比。

he expression of integrin Alpha-6 subunit,Iaminin,type IV collagenase,type IV collagen and ras p21 were studied immunohistochemically in gastric cancer. The results showed that the expression of alpha 6 and laminin(LN)was often in continuous or interrupted linear pattern in the expanding type of gastric carcinoma(GC);while in the infiItrating type of GC,they were expressed either in interrupted spotty or fragmentary pattern, or almost lost. Suggesting that the interaction between the laminin receptor and its ligand may influence the mode of growth in GC. Intense expression of type IV collagenase was often found in GC cells,especially in the infiltrative type of GC and in those with lymph node metastasis,it can therefore be used as a marker for invasion and metastasis of GC cells.A positive correlation was found between the expression of type IV collagenase and ras p21 in GC. The expression of type IV collagen was similar to that of laminin,but in reverse proportion to the expression of type IV collagenase. These investigations provide a better understanding of the molecular pathological basis of tumor invasion and metastasis.