焦化废水中细菌质粒的研究

Study on the plasmids of the bacteria in the coke wastewater

细菌质粒中常带有一些可编码降解特殊有毒物质酶的基因,为了研究质粒对有毒物质CN-的降解的意义,主要调查了焦化废水中好氧异养菌的质粒分布特点。从山西省焦化企业公司生化站、太原煤气公司焦化厂生化站中筛选出53株细菌,利用琼脂糖凝胶电泳法,采用GDS8000型凝胶电泳分析仪进行拍照,同时测定各菌株降氰、降酚能力,CN-采用异烟酸吡唑啉酮法,酚采用4氨基安替比林法测定。结果表明,质粒的存在与降氰力有一定的关系,但对降酚力的影响差异不显著。同时,通过对其中11#号菌株进行了质粒转化和消除实验,证明质粒稳定,不可用十二烷基磺酸钠(SDS)消除掉,用E.ColiDHI作受体菌,用11#菌株作供体菌,作转化实验,但由于种种原因,没有筛选到转化子。

The bacteria often carry some genes that could produce some enzymes to degrade toxic substance. In order to study the relationship between the degradation rate and plasmid, the 53 bacteria isolated from cyanide-containing area have been tested whether they had plasmid.Results showed that plasmid was very important for bacteria in cyanide-containing areas,but inconspicuousness or unimportance for hydroxybenzene-degrading. No.11 isolate was selected from 53 isolates, an effective cyanide-degrading isolate, and tried to dissolve the plasmid of it,but the plasmid was very difficult to dissolve. In addition, it failed to transform No.11 isolate into E.Coli DHI, maybe because the size of the plasmid was too great.