摘要
本研究将特异性免疫核糖核酸(i-RNA)用于巨细胞病毒(CMV)感染母亲所生的婴儿,采用四甲基偶氮唑盐(MTT)比色法,测定了治疗前后白细胞介素2(IL-2)的活性。结果:治疗组25例,IL-2活性分别为1.39±0.17GI和1.60±0.21GI(P<0.01);对照组10例,IL-2活性分别为1.47±0.14GI和1.38±0.20GI(P>0.05)。两组比较差异有显著性(P<0.01),提示特异性i-RNA对新生儿外周血IL-2活性有明显的增强作用。作者同时对35例受试对象进行了2年的定期随访,结果:治疗组25例,无1例发生巨细胞包涵体病(CID),对照组10例中有2例发病。本研究为临床应用特异性i-RNA预防CMV母婴垂直感染提供了实验依据。
With the method of MTT colorimetery,the effect of immune RNA(i-RNA )oninterleukin-2(IL-2)activity of newborn infants born to mothers with cytomegalovirus(CMV)infection was tested.The results showed that in the i-RNA treated group(n=25),IL-2 activity before and after therapy was l37±9.17GI and 1.60 ±0.21 GI,respectirely(P<0.01);while in the control group(n=10),the IL-2 activity was1.47±0.14 GI and 1.38±0.20 GI, respectively significant change(P>0.05).Therewas marked difference in IL-2 activity in the two groups(P<0.01).It is indicatedthat i-RNA can enhance IL-2 activity of newborn infants.This study providestheoretical basis for the use of i-RNA to prevent CMV vertical infection from mother-toinfants.
出处
《中华传染病杂志》
CSCD
北大核心
1994年第3期153-156,共4页
Chinese Journal of Infectious Diseases
基金
卫生部
关键词
巨细胞病毒
垂直传播
IL-2
IRNA
Cytomegalovirus infection
Vertical transmission
Immune RNA
Inter- leukin-2