摘要
本研究将特异性免疫核糖核酸（ｉ－ＲＮＡ）用于巨细胞病毒（ＣＭＶ）感染母亲所生的婴儿，采用四甲基偶氮唑盐（ＭＴＴ）比色法，测定了治疗前后白细胞介素２（ＩＬ－２）的活性。结果：治疗组２５例，ＩＬ－２活性分别为１．３９±０．１７ＧＩ和１．６０±０．２１ＧＩ（Ｐ＜０．０１）；对照组１０例，ＩＬ－２活性分别为１．４７±０．１４ＧＩ和１．３８±０．２０ＧＩ（Ｐ＞０．０５）。两组比较差异有显著性（Ｐ＜０．０１），提示特异性ｉ－ＲＮＡ对新生儿外周血ＩＬ－２活性有明显的增强作用。作者同时对３５例受试对象进行了２年的定期随访，结果：治疗组２５例，无１例发生巨细胞包涵体病（ＣＩＤ），对照组１０例中有２例发病。本研究为临床应用特异性ｉ－ＲＮＡ预防ＣＭＶ母婴垂直感染提供了实验依据。
With the method of MTT colorimetery,the effect of immune RNA(i-RNA )oninterleukin-2(IL-2)activity of newborn infants born to mothers with cytomegalovirus(CMV)infection was tested.The results showed that in the i-RNA treated group(n=25),IL-2 activity before and after therapy was l37±9.17GI and 1.60 ±0.21 GI,respectirely(P<0.01);while in the control group(n=10),the IL-2 activity was1.47±0.14 GI and 1.38±0.20 GI, respectively significant change(P>0.05).Therewas marked difference in IL-2 activity in the two groups(P<0.01).It is indicatedthat i-RNA can enhance IL-2 activity of newborn infants.This study providestheoretical basis for the use of i-RNA to prevent CMV vertical infection from mother-toinfants.
出处
《中华传染病杂志》
CSCD
北大核心
1994年第3期153-156,共4页
Chinese Journal of Infectious Diseases
基金
卫生部
关键词
巨细胞病毒
垂直传播
IL-2
IRNA
Cytomegalovirus infection
Vertical transmission
Immune RNA
Inter- leukin-2
