聚合酶链反应快速诊断流行性脑脊髓膜炎

RAPID DIAGNOSIS OF MENINGOCOCCAL MENINGITIS BY POLYMERASE CHAIN REACTION

采用聚合酶链反应（ＰＣＲ）扩增脑膜炎奈瑟氏菌（Ｎｍ）ＩＳ１１０６插入序列的方法诊断流行性脑脊髓膜炎（流脑）。所试２１株Ｎｍ均扩增产生预期长度５９６ｂｐ的特异性片段，１４株非Ｎｍ菌株未见此片段。扩增灵敏度为１２ｆｇ。Ｎｍ菌ＩＳ１１０６重复序列扩增产物构成特征性ＰＣＲ指纹图，Ａ群菌株图谱一致；Ｂ群菌株呈明显多态性变化。临床标本的ＰＣＲ检测结果为：２１份流脑患者脑脊液中２０份为阳性（９５．２％）；１４份流脑患者急性期血清中１２份阳性（８５．７％）；在所检标本中Ｎｍ培养阳性的８份脑脊液和４份急性期血清ＰＣＲ检查也同样阳性。作为对照的１２份恢复期血清、３份密切接触者血清和２０份正常人血清均为阴性。作者认为，ＩＳ１１０６－ＰＣＲ是诊断流脑的一种简便快速、灵敏特异的方法。

olytnerase chain reaction(PCR)was used for diagncsis of meningococcal menin-gitis by amplification of ISII06 insertion sequence of N。meningitidis.All 2l strainsof N。meningitidis yielded a specific 596bp fragment but l4 non-meningococcal strainsdidn't.The sensitivity of amplification was about 12fg.PCR amplified fingerprintswere generated from the repetitious sequence of ISII06.The band patterns ofmeningococcal strains groups A were very much simil ar but those of group B strainswere quite diverse.With aid of the ISII06-PCR,20 of the 2l cerebrospinal fluids(CSF)Specimens and 12 of the l4 acute stage sera of suspected patients were PCR-positive.In the above samples tested,8 CSF and 4 sera were from patients with pro-ven meningococcal meningitis and they were also PCR-positive,In order to confirmthe specificity of the ISII06-PCR,12 convalescent sera,3 close contacts’ Sera and 20normal sera were examined and they were all PCRnegative。The results of the presentstudy suggest that ISII06-PCR is a sensitive and specific method for rapid diagnosisof meningococcal meningitis。