Zn、Mg对大鼠成骨细胞生长分化的影响

Effect of Trace Elements Zn, Mg on Osteoblast Growth and Differentiation in Vitro

利用Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ鼠胚颅骨酶消化分段收集原代成骨细胞，在培养物中加入微量元素，进行细胞形态学观察和分泌功能鉴定来阐明Ｚｎ、Ｍｇ在骨折愈合过程中的代谢情况。实验结果表明：微量元素剂量不同可对成骨细胞功能产生双相作用，Ｚｎ１×１０－７ｇ／ｍｌ、Ｍｇ１×１０－５ｇ／ｍｌ促进成骨细胞碱磷酶活性及蛋白质分泌，浓度太高太低都适得其反。另外，笔者还观察到微量元素对成骨细胞均有一个最佳浓度效应，认为微量元素应用于临床，对加速骨折愈合具有重要意义。

Osteoblasts were collected from the calvaria of spragua Dawley rat embryos in which the cell were digested by collagenase. The cell’s morphology and it’s secretory functions were investigated. After cultured 1￣2 weeks, it was diseovered that the conditioned medium Zn 1×10 7 ,Mg 1×10 5 g/ml could stimulates the synthesis of cell proteins and elevate the activity of alkaline phosphatase,and each trace element had an optimum effective concentration response to the osteoblasts.