实验性脑损伤后脑皮质及血浆儿茶酚胺含量的改变

Changes of Catecholamine in Brain Cortex and Plasma Following Experimental Brain Injury

采用大鼠脑损伤模型，高效液相色谱法检测伤后６、２４、７２及１６８小时脑皮质及血浆儿茶酚胺（ＣＡ）含量。结果表明，脑皮质ＣＡ含量在伤后６小时显著升高，去甲肾上腺素（ＮＥ）、肾上腺素（Ｅ）及多巴胺（ＤＡ）分别为对照组的２００％（Ｐ＜０．０１）、２９５％（Ｐ＜０．００１）和１２６％（Ｐ＜０．０５），其后迅速下降，于伤后７２小时３种ＣＡ含量均明显低于正常值（Ｐ＜０．０５）。血浆ＣＡ含量也于伤后６小时达峰值，ＮＥ、Ｅ及ＤＡ分别达对照组的３３１％（Ｐ＜０．０１），７４０％（Ｐ＜０．００１）和１８０％（Ｐ＜０．０５），以后缓慢回降，至伤后１６８小时ＮＥ、Ｅ含量仍明显高于正常值（Ｐ＜０．０５）。对脑损伤后ＣＡ变化的机理及其对继发性脑损害的影响进行了讨论。

In the present study, the contents of catecholamine (CA) in brain cortex and plasma were determined by the high performance liquid chromatography at 6, 24, 72 and 168 hours after experimental brain injury in rats. Dramatic rises of CA contents in ipsilateral cerebral cortex were found at 6 hours postinjury. Norepinephrine (NE), epinephrine (E) and dopamine (DA) increased to about 200% ( P <0.01), 295% ( P <0.001) and 126% ( P <0.05) of their control values respectively. Then they declined rapidly and significantly lowered than normal values ( P <0.05) at 72 hour after trauma. Plasma CA demonstrated a nearly 331% ( P <0.01), 740% ( P <0.001) and 180% ( P <0.05) increase in NE, E and DA respectively at 6 hour following injury, and remained at high levels over the period of the study.The mechanism of the alterations of CA contents following brain injury and the influence of elevated CA level on the secondary brain damage are discussed in this paper.