四种移植材料修复兔颅骨缺损的比较研究

A Comparison of Healing in Four Graft Materials in Rabbit Skull Defects

首次应用人脱蛋白骨（ｈ－ＤＰＢ）、人脱蛋白骨／骨形成蛋白（ｈ－ＤＰＢ／ＢＭＰ）、羟基磷灰石（ＨＡＰ）、ＨＡＰ／ＢＭＰ作比较，修复兔颅顶骨缺损并探讨其成骨差异。７８只大白兔被随机匀分Ａ、Ｂ两组，在兔颅顶骨两侧对称各制造直径为１ｃｍ的圆形骨缺损，将按实验要求制备好的ｈ－ＤＰＢ／ＢＭＰ、ＨＡＰ／ＢＭＰ、ｈ－ＤＰＢ、ＨＡＰ分别植入Ａ组和Ｂ组兔左右骨缺损内。移植材料内的３５Ｓ、４５Ｃａ和灰量的测定结果及组织学、Ｘ光摄片检查结果表明，四种移植材料的成骨能力依次是：ｈ－ＤＰＢ／ＢＭＰ＞ＨＡＰ／ＢＭＰ（Ｐ＜０．０５）＞ｈ－ＤＰＢ和ＨＡＰ（Ｐ＜０．０１），而ｈ－ＤＰＢ和ＨＡＰ无显著性差异（Ｐ＞０．０５）。认为，ｈ－ＤＰＢ来源丰富，成本低，比ＨＡＰ更适合作ＢＭＰ的载体，ｈ－ＤＰＢ／ＢＭＰ是目前较理想的骨移植材料。

This paper reports the difference between human deproteined bone (h-DPB),composite of h-DPB and bone morphogenetic pro-tein (h-DPB /BMP).composite Hydroxyapatite(HAP/BMP)and HAP in repairing rabbits skull bone defects. Seventy-eight adultwhite rabbits were averagely divided into A and B groups at random. The experimental models were made by trephined 1 cm circulardefects in the both parietal bone of each rabbit. Each rabbit bone defects in A group were grafted into one h-DPB/BMP and oneHAP/BMP in B group one h-DPB and one HAP, The35 S、45Ca contents and ash weight of the implants were measured and theirroentgenological and histological changes were observed. The results showed, that the osteogenesis of the graft materials was h-DPB/BMP>HAP/BMP(P<0.05)>h-DPB and HAPP<0.01).No statistical defferences were found between h-DPB and HAP.We conclude that deproteined bone are better as a carrier of BMP than HAP, and worthy to be recommended for clinical use.