血清触珠蛋白在烧伤后B淋巴细胞功能紊乱中的作用

Role of Serum Haptoglobin in Postburn B-lymphocyte Dysfunction

旨在进一步观察烧伤后血清触珠蛋白（ＨＰ）对Ｂ淋巴细胞功能的影响，以期弄清血清ＨＰ在烧伤后免疫功能紊乱发生机制中的作用。先采用亲和层析法从烧伤血清纯化ＨＰ，然后在培养的正常小鼠脾细胞中分别按正常血清ＨＰ含量（火箭电泳法：１．２３３ｍｇ／ｍｌ）和烧伤血清ＨＰ含量（火箭电泳法：９．４０７ｍｇ／ｍｌ）加入纯化ＨＰ，观察ＨＰ对美洲商陆（ＰＷＭ）和脂多糖（ＬＰＳ）诱导的脾细胞ＩｇＧ产生能力的影响，用ＥＬＩＳＡ检测培养细胞上清ＩｇＧ含量。结果表明，相当于烧伤血清ＨＰ含量的纯化ＨＰ对ＬＰＳ诱导的脾细胞ＩｇＧ产生无明显影响，却显著抑制ＰＷＭ诱导的脾细胞ＩｇＧ产生；而相当于正常血清ＨＰ含量的纯化ＨＰ对ＰＷＭ和ＬＰＳ诱导的脾细胞ＩｇＧ产生均无明显影响。提示，烧伤后血清高浓度的ＨＰ可能通过抑制Ｔ淋巴细胞和／或其淋巴因子的功能而间接影响Ｂ淋巴细胞，从而参与烧伤后免疫功能紊乱的发生与发展。

he mechanism of postburn immunosuppression has not yet been clarified, but it is well known that serum immunosuppressivefactors play an important role in the pathogenesis of the disturbance of immune response following burn injury. In our previousstudy, it was found that serum haptoglobin exhibited severe immunosuppressive effect on T-lymphocyte function following burns, sothis study was intended to investigate the effect of haptoglohbin purified from the serum of burned patients by affinity chromatogra-phy,thus clarifying to the role of serum haptoglobin in postburn B-lymphocyte dysfunction. The results showed that purified hap-toglobin, at the level equivalent to the concentration found in serum of burned patients, significantly inhibited immunoglobulin G(IgG) synthesis induced by pokeweed mitogen (PWM),but did not affect IgG synthesis induced by lipopolysaccharide (LPS). Fromthese result, it is suggested that extraordinary increase in serum haptoglobin following burn may play a role in the pathogenesis ofB-lymphocyte dysfuntion by suppressing T-lymphocyte function.