钙与新生大鼠缺氧缺血性脑病

CHANGES OF CALCIUM CONTENT INHYPOXIC-ISCHEMIC ENCEPHALOPATHY OFNEWBORN RATS

结扎７日龄Ｗｉｓｔａｒ大鼠左侧颈总动脉后吸入８％浓度氧２小时，制成缺氧缺血性脑病模型。４４小时后测定各组动物脑组织Ｃａ￣（＋＋）含量。结果正常对照组、缺氧组、缺血组脑Ｃａ￣（＋＋）含量相似；缺氧缺血组脑Ｃａ￣（＋＋）含量明显高于上述三组；丹参预防组和治疗２组脑Ｃａ￣（＋＋）含量比缺氧缺血组明显降低。提示缺氧缺血后Ｃａ￣（＋＋）在脑组织中聚集，丹参注射液能降低缺氧缺血后脑Ｃａ￣（＋＋）含量，预防性给药效果优于缺氧缺血后给药。

AbstractModels of hypoxic-ischemic eneephalopathy ofnewborn rats were established after ligation of leftcarotid artery and-inhalation of 8% oxygen for 2hours in 7-day old newborn rats. 44 hours afterligation caicium content of control, hypoxic, isehemic,hypoxic-ischemic, prevention, lst treatment and 2ndtreatment group was detennined. The value in thehypoxic-ischemic groups was much higher than thatof the first three groups ainong which the values weresimilar. Calcium contents in prevention and 2ndtreatment groups with injeetio salviae miltiorrhizaewere significantly lower than that of hypoxic-ischemicgroups. This study demonstrated that (1) afterhypoxic-ischemic damage accumulation of calcium inbrain tissue occurredi (2) injectio salviae miltiorrhizaecould reduee calcium accumulation, especially beforeoccurrenee of hypoxic-ischemic demage.