聚合酶链反应在肺炎支原体检测中的临床应用与研究

A STUDY ON CLINICAL APPLICATION OFPOLYMERASE CHAIN REACTION IN DETEC-TION OF MYCOPLASMA PNEUMONIAE

采用聚合酶链反应结合分子杂交技术对多种已知支原体、模似临床标本、临床标本，以及动物模型进行研究，建立了一套可在２小时内对肺炎支原体（ＭＰ）感染作出诊断的实验方法，结果表明，此方法对ＭＰ具有高度特异性，与其它支原体无交叉反应，检测灵敏度可达１ｃｃｕ（相当于１０～４０个ＭＰ）。检测了２００例临床病例标本，有３５例阳性，与血清学方法基本相符。此方法简便、快速、成本低廉，适合临床应用。

AbstractIt is important to develop rapid sensitive and spe-cific methods for the diagnosis of mycoplasmapncumonial infection in order to facilitate early anti-biotic treatment. We used the polymerase chain reac-tion (PCR) to detect the mytoplasma pneumoniae. Wefirst set up the animal model by inoculatingmycoplasma pneumoniae to sunulate the process ofmycoplasma pneumoniae infection in human body;each animal inoculated gave a positive result in bothculture and PCR: by which I CCU of mycoplasmapneumoniae could be detected. PCR was positive in 35of the 200 cases with acute respira-tory symptoms:serological tests also confinned that most of the 35cases had acute mycoplasma pneumoniae infections.The procedures of the PCR, including sample collec-tion and proeessing, can be finished with 2 hours.