纤维素分解菌的筛选及鉴定

ISOLATION AND IDENTIFICATION OF CELLULOSE-DECOMPOSING MIXED STRAIN

从自然界中筛选分离获得具有高效分解纤维素功能的一组混合菌株,经分离纯化得3个菌株D1、D2和D3,利用16SrDNA测序鉴定,这些菌株分别是克雷伯氏菌、假单胞菌和嗜麦芽窄食单胞菌,比较各单个菌株及其相互混合的羧甲基纤维素(CMC)酶相对活性、以及它们对滤纸和香蕉杆的分解效果,发现各单一菌株对纤维素类物质均有一定的降解效果,但混合菌群效果最好,其CMC酶相对活性为0.93cm/d,说明多种微生物的协同作用有助于纤维素类物质分解。

A cellulose-decomposing mixed strain was isolated from natural habitats, which contained Klebsiella sp., Pseudomonas sp., Stenotrophomonas sp. identificated by 16S rDNA sequencing. By comparing the relative activity of carboxymethyl cellulose(CMC)enzyme and the effects on decomposing filter paper and banana plant stalks among each single bacteria and the mixed strain, it was shown that each strain had certain capability of decomposing cellulose, but mixed strain behaved the best and the relative activity of CMC enzyme was 0.93 cm/d. It indicated that synergistic effect among several microorganisms would accelerate the decomposition of cellulose materials.