肝组织中糜酶浓度在慢性肝炎肝纤维化中的意义

Hepatic chymase content in chronic hepatitis and hepatic fibrosis

为探讨肝组织中糜酶(Chymase)浓度在慢性病毒性肝炎肝纤维化中的意义。采用酶联免疫吸附实验(ELISA)检测45例慢性肝炎肝组织中Chymase浓度,观察不同的纤维化分期(S1-S4),其肝组织中Chymase浓度;用免疫组织化学染色法观察Chymase单克隆抗体标记的肥大细胞分布情况。慢性肝炎纤维化分期重的S3和S4患者,其肝组织中Chymase浓度〔S3+S4=(31.3±24.6)ng/mg〕明显高于纤维化轻的S1和S2患者〔S1+S2=(5.7±4.8)ng/mg〕,P<0.01;免疫组化染色结果,Chymase标记的肥大细胞主要分布于纤维化旺盛的汇管区与类洞壁,其分布与纤维化部位相一致,且肝组织中Chymase浓度高的患者,其肝组织内Chymase标记的肥大细胞分布增多。推测肝组织中Chymase浓度可能与慢性肝炎肝纤维化关系密切。

To investigate spatial relatioship betwwen human chymase and fibrosis in liver specimens affected by chronic hepatitis.The chymase levels were ditermined by using the method of enzyme-linked immunosorbant assay (ELISA) in 45 cases of chronic hepatitis.Mast cell distribution in the specimens was determined immunohistochemically by using anti-chymase antibody.Hepatic chymase concentrations in S3 and S4 cases were greater than in S1 and S2 ,〔S3+S4=(31.3±24.6)ng/mg vs.S1+S2=(5.7±4.8)ng/mg,P<0.01〕.Cells immunoreactive for chymase were seen throughout portal areas and intralobular sinusoidal walls,largely colocalizing with flbrosis.Chymase appears to be involved in hepatic fibrosis in chronic hepatitis.