小檗碱-HAuCl_4-KI体系的光度法研究及应用

SPECTROPHOTOMETRIC STUDY OF BERBERINE-HAuCl_4-KI AND ITS ANALYTICAL APPLICATION

采用分光光度法研究了小檗碱-氯金酸-卤化钾等5个缔合微粒体系.结果表明,4.0×10-2mol/LHCl-2.8×10-3mol/LKI-2.4×10-4mol/LHAuCl4体系的最大吸收峰在370nm,当有盐酸小檗碱(BH)存在时,由于形成(BH-AuI2)n缔合微粒,在550nm处有一吸收峰,BH浓度在0.16～24mg/L范围遵循Lamber-Beer定律,其摩尔吸光系数ε550nm为1.71×104L·mol-1·cm-1.据此建立了一个测定中药及其制剂中的盐酸小檗碱含量的分光光度法,平均回收率为100.5%,相对标准偏差RSD为1.00%(n=5).

Five association particle systems of berberine-HAuCl_4-KX were studied spectriphotometrically.There is a max absorption peak at 370 nm for the system of 4.0×10^(-2) mol/L HCl-2.8×10^(-3) mol/L KI-2.4×10^(-4) mol/L AuCl^-_4.As berberine hydrochloride (BH) co-exists,the formation association particles of (BH-AuI_2)_n exhibit a new absorption peak at 550 nm.BH concentration is obeyed Lamber-Beer law in the range of 0.16～24 mg/L.Its mole absorption efficient ε_(550 nm) is 1.71×10~4 L·mol^(-1)·cm^(-1).A new spectrophotometric method has been proposed for the determination of BH in real samples,with some advantages including high sensitivity,simplicity,rapidity and accuracy.The means recovery is 100.5%.The RSD is 1.00% (n=5).