适用于SSR分子标记的玉米单粒种子DNA快速提取新方法

A New Rapid DNA Extraction Method from Maize Single Dry Seed Suitable for SSR Analysis

研究了玉米单粒种子DNA的提取新方法,利用该方法提取DNA,液氮、研磨、离心、沉淀、SDS、CTAB及氯仿都不用,一个工作人员提取100粒种子(1个检测样品)DNA只需30min左右,一天可以提取1600粒种子(16个检测样品)的DNA,并且提取的DNA分子量大,不降解,完全可以满足利用SSR分子标记进行DNA指纹分析的需要,为DNA指纹技术在玉米种子纯度及真伪快速鉴定中的广泛应用解决了关键性技术难题。

A new rapid DNA extraction method from maize single dry seed was s tudied. It needed no liquid nitrogen, no grinding, no centrifuged, no precipitat ed, no SDS, no CTAB, no chloroform, et al. It costs about 30 minutes to extract DNA from each of 100 dry seeds, and in a single day, one person can complete DNA isolation from more than 1600 individuals, and the quality of DNA extracted usi ng this method was suitable for SSR analysis. It resolved the critical technique problem of DNA fingerprint widely used in identification of maize seed purity.